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10.13863/j.issn1001-A4454.2010.09.018 80 GeorgeK

elegans is associated with piRNAs, 22G-RNAs, and histone H3K9me3 and H3K27me3 marks 11,35,86,114

For single sgRNA cloning, the oligonucleotides containing 20 bp protospacer and overhang were obtained from Integrated DNA Technologies and cloned into the Bsm BI-v2 (New England Biolabs, catalogue no